The mechanical properties of microscopic particles have been a heated research object for it takes the deformation of micro-beads in the microfluidic environment into account. Sufficient knowledge on mechanical properties of micro-beads would lead to better device design and application for cell mechanics, tissue engineering, etc. The physical properties of alginate beads were examined both in normal condition and under compression, to illustrate its mechanical stability and to calculate the shear modulus through Hertz model. Furthermore, the modeling of physicochemical variation of micro-beads under the ultrasonic thermal effect was performed. The temperature rose simultaneously with ultrasonic thermal effect. The shear module and diameter of micro-beads changed with the increase of temperature in the solution. The descriptive model and the predictive model for the relationship between temperature and the module/diameter of micro-beads were established, and the validation process presented the effectiveness of the models.
 M.Ø. Dalheim, J. Vanacker, M.A. Najmi, F.L. Aachmann, B.L. Strand, B.E. Christensen, “Efficient functionalization of alginate biomaterials”, J. Biomater., Vol. 80, 2016, pp. 146-156.
 J.Y. Leong, W.H. Lam, K.W. Ho, W.P. Voo, M.F.X. Lee, H.P. Lim, S.L. Lim, B.T. Tey, D. Poncelet, E.S. Chan, “Advances in fabricating spherical alginate hydrogels with controlled particle designs by ionotropic gelation as encapsulation systems”, Particuol., Vol. 24, 2016, pp. 44–60.
 D. Portnikov, H. Kalman, “Determination of elastic properties of particles using single particle compression test” Powder Technol., Vol. 268, 2014, pp. 244-252.
 E.A. Peeters, C.W. Oomens, C.V. Bouten, D.L. Bader, F.P. Baaijens, “Mechanical and failure properties of single attached cells under compression”, J. Biomech., Vol. 38(8), 2005, pp. 1685-1693.
 A. Overbeck, I. Kampen, A. Kwade, “Mechanical characterization of yeast cells: Effects of growth conditions”, Lett. Appl. Microbiol., Vol. 61(4), 2015, pp. 333-338.
 X.Y. Tian, X.B. Chen, “Effects of Cell Density on Mechanical Properties of Alginate Hydrogel Tissue Scaffolds”, J. Biomim. Biomater. Tissue Eng., Vol. 19, 2014, pp. 77-85.
 C.X. Wang, C. Cowen, Z. Zhang, C.R. Thomas, “High-speed compression of single alginate microspheres”, Chem. Eng. Sci., Vol. 60(23), 2005, pp. 6649–6657.
 S.T. Moe, K.I. Draget, G.S. Bræk, O. Simdsrød, “Temperature dependence of the elastic modulus of alginate gels”, Carbohydr. Polym., Vol. 19(4), 1992, pp. 279-284.
 D. Serp, M. Mueller, U.V. Stockar, I.W. Marison, “Low-Temperature Electron Microscopy for the study of polysaccharide ultrastructures in hydrogels II effect of temperature on the structure of Ca2+ alginate beads”, Biotechnol. Bio. Eng., Vol. 79, 2002, pp. 253-259.
 M.A. Tung, M.D.H. Rogers, General Compressive Measurements, in Current Protocols in Food Analytical Chemistry, by John Wiley and Sons, Inc. 2001, H2.1.1-H2.1.8.
 B. David, E. Dore, M.Y. Jaffrin, C. Legallais, “Mass transfers in a fluidized bed bioreactor using alginate beads for a future bioartificial liver”, Int. J. Artif. Organs., Vol. 27, 2004, pp. 284-93.
 A.V. Salsac, L. Zhang, J.M. Gherbezza, “Measurement of mechanical properties of alginate beads using ultrasound”, 19eme Congrees de Mecanique, France, 2009, pp. 1-6.
 S. Vicini, M. Castellano, M. Mauri, E. Marsano, “Gelling process for sodium alginate: New technical approach by using calcium rich micro-spheres”, Carbohydr. Polym., Vol. 134, 2015, pp. 767–774.
 E. Favre, M. Leonard, A. Laurent, E. Dellacherie, “Diffusion of polyethyleneglycols in calcium alginate hydrogels”, Colloids Surf., A, Vol. 194(1-3), 2001, pp. 197–206.