Immobilization of Glucose oxidase on Meso-porous Glass-ceramic with the Skeleton of CaTi4(PO¬4)6

Document Type: Research Paper

Authors

1 Department of Mechanical Engineering, school of material Engineering, University of Tabriz, Tabriz, Iran.

2 Department of Chemistry, University of Tabriz, Tabriz, Iran.

3 Department of Ceramics, Materials and Energy Research Center, Karaj, Iran.

Abstract

Microporous glass ceramic with skeleton of CaTi4(PO¬4)6 with average pore size of 12.7 nm has been synthesized and used as a carrier of glucose oxidase. The glass ceramic was prepared by controlled heat treatment of glass samples, which causes the phase separation in their structure and creates CaTi4(PO¬4)6 and β-Ca3(PO4)2 phases. The β-Ca3(PO4)2 phase was dissolved by soaking the glass ceramics in HCl and CaTi4(PO¬4)6 built the skeleton of microporous glass ceramic. Analysis of the ability of the carrier for immobilization of glucose oxidase (GOx) was undertaken. Average amount of immobilized enzyme and percentage of enzyme activity on the carrier were 27 gr GOx/gr carrier and 60.15%, respectively. Effect of pH and temperature variations on the enzyme activity has been studied and results demonstrated that maximum activity for both free and immobilized enzyme was at T=40˚C and pH=7.0. Due to the same value of maximum activity, no serious conformational change of enzyme had taken place through immobilization. However, immobilization of GOx on CTP caused to considerable increase of enzyme stability under different environmental conditions.

Keywords

Main Subjects


  1. B. M. Brena, F. B. Viera,Immobilization of enzymes, In:J.M. Guisan (Ed.), Immobilization of Enzymes and Cells, springer, Germany, 2006, pp. 15-29.
  2. C. Spahn, S. D. Minteer,“Enzyme Immobilization in Biotechnology”, Recent patents on engineering 2, 2008, 195-200.
  3. O. Kirk, T. V. Borchert, C. C. Fuglsang,“Industrial enzyme applications”, Current opinion in biotechnology, Vol. 13, 2002,pp. 345-351.
  4. T. Tosa, T. Sato, T. Mori, Y. Matuo, I. Chibata,“Continuous production of L-aspartic acid by immobilized aspartase”, Biotech. Bioeng.,Vol. 15, 1975,pp. 69-84.
  5. T. Suzuki, M. Toriyama, H. Hosono, Y. Abe,“Application of a microporous glass-ceramic with a skeleton off CaTi4(PO4)6 to carriers for immobilization of enzymes”, J. fermentation bioeng.,Vol. 72, 1991,pp. 384-391.
  6. C. C. Ribeiro, C. C. Barrias, M. A. Barbosa,“Calcium phosphate-alginate microspheres as enzyme delivery matrices”, Biomat., Vol. 25, 2004, pp. 4363-4373.
  7. J. Kim, J. W. Grate, P. Wang,“Nanostructures for enzyme stabilization”, Chem. Eng. Sci.,Vol. 61, 2006, pp. 1017-1026.
  8. A. E. David, N. S. Wang, V. C. Yang, A. J. Yang,“Chemically surface modified gel (CSMG): An excellent enzyme-immobilization matrix for industrial processes”, J. Biotech.,Vol. 125,2006, pp. 395-407.
  9. J. Raba, H. A. Mottola,“Glucose Oxidase as an Analytical Reagent”, Critical Reviews in Analytical Chemistry, Vol. 25, 1995, pp. 1-42.
  10. H. Hosono, Y. Abe,“Porous Glass Ceramics Composed of a Titanium Phosphate Crystal Skeleton: A Review”, J.. Non-Cryst. Solid, Vol. 190, 1995, pp. 185-197.
  11. V. Bulmuş, H. Ayhan, E. Pişkin,“Modified PMMA monosizemicrobeads for glucose oxidase immobilization”, Chem. Eng. J., Vol. 65, 1997, pp. 71-76.
  12. N. V. Klassen, D. Marchington, H. C. E. McGowan,“H2O2 determination by the I-3 Method and by KMnO4 Titration”, Analy. Chem., Vol. 66, 1994, pp. 2921-2925.
  13. S. Kalayc, G. Somer, G. Ekmekci,“Preparation and application of a new glucose sensor based on iodide ion selective electrode”, Talanta, Vol. 65, 2005, pp. 87-91.
  14. F. Soleimani, M. Rezvani,“The effect of CeO2 addition on crystallization behavior and pore size in microporous calcium titanium phosphate glass ceramics”,Materials Research Bulletin, Vol. 47, 2012, pp.
    1362-1367.
  15. C. S. Ray, X. Fang, D. E. Day, “New Method for Determining the Nucleation and Crystal-Growth Rates in Glasses”, J. Am.Ceram. Soc., Vol. 83, 2000, p.865.
  16. M. Gerard, A. Chaubey, B. D. Malhotra, “Application of conducting polymers to biosensors”, Biosensors and Bioelectronics, Vol. 17, 2002, pp. 345-359.
  17. F. M. Bautista, J. M. Campelo, A. Garcia, A. Jurado, D. Lunda, J.M. Marinas, A. A. Romero, “Acetonylacetone conversion on AlPO4–cesium oxide (5–30 wt%) catalysts”, J. Mol. Catalysis B: Enzymatic, Vol. 11, 2001, p. 567.
  18. L. C. Dong, G. Wang, Y. Xiao, Y. Xu, X. Zhou, H. Jiang, Q. Luo, “Immobilization of Glucose Oxidase on a Novel Crosslinked Chitosan Support Grafted With L-Lysine Spacers”, Chem. Biochem.Eng., Vol. 25, 2011, pp. 395-402.
  19. B. R. Azamian, J. J. Davis, K. S. Coleman, C.B.Bagshaw, M.L.H. Green, “Bioelectrochemical Single-Walled Carbon Nanotubes”, J. Am. Chem. Soc. Vol. 124, 2002, pp. 12664-12665.
  20. S. Rauf, A. Ihsan, K. Akhtar, M. A. Ghauri, M. Rahman, M. A. Anwar, A. M. Khalid, “Glucose oxidase immobilization on a novel cellulose acetate–polymethylmethacrylate membrane”, J. Biotech., Vol. 121, 2006, pp.351-360.
  21. X. Hou, B. Liu, X. Deng, B. Zhang, H. Chen, R. Luo,“Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization”, Analytical Biochem., Vol. 368, 2007, pp.100-110.